The method consists in hydrolysing with hydrochloric acid of a ground test portion, dollowed by separation of the total lysine by column chromatography and determination by spectrometry at 570 nm. Reaction of a second ground test portion with an ethanolic solution of 2,4-dinitrofluorobenzene in an alkaline medium, purification, then hydrolysis with hydrochloric acid, separation of the non-available lysine by column chromatography, and determination by spectrometry at 570 nm.
Life cycle
NOW
PUBLISHED ISO 5510:1984 90.93
Standard confirmed 11 dhj 2021
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