Apple juice, apple juice concentrates and drinks containing apple juice — Determination of patulin content — Part 2: Method using thin-layer chromatography
The principle of the method specified is extraction of patulin in a mixture of ethyl acetate and chloroform (3:2 by volume), filtration of the extract on a silica-gel column and qualitative and quantitative determination by means of two-directional thin-layer chromatography. The spots are developed using a 3-methyl-2-benzothiazoline hydrazone (MBTH) hydrochloride solution. The limit of detection is 25 µg/l, based on 50 ml of ready-to-drink apple juice. The more precise method of analysis is HPLC.
Life cycle
NOW
PUBLISHED ISO 8128-2:1993 90.93
Standard confirmed 20 jan 2022
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